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Review
11.
Molecular diagnosis of haemorrhagic septicaemia -
A review - Ranjan Rajeev, S. K. Panda, A. P. Acharya, A. P.
Singh, M. K. Gupta
Vet World. 2011; 4(4): 189-192
doi:
10.5455/vetworld.2011.189-192
Abstract
Pasteurella multocida is associated with
hemorrhagic septicaemia in cattle and buffaloes,
pneumonic pasteurellosis in sheep and goats, fowl
cholera in poultry, atrophic rhinitis in pigs and
snuffles in rabbits. Haemorrhagic septicaemia is
caused by Pasteurella multocida type B:2, B:2,5
and B:5 in Asian countries and type E:2 in African
countries. Pasteurella multocida have five types
of capsular serotype i.e. type A, B, D, E and F.
Diagnosis of the disease is mainly based on the
clinical sign and symptom, post mortem findings.
Confirmatory diagnosis is done by isolation and
identification of causative agent. A variety of
laboratory diagnostic techniques have been
developed over the years for pasteurellosis and
used routinely in the laboratory. Among these
techniques molecular techniques of diagnosis is
most important. This technique not only gives
diagnosis but it also provides information
regarding capsular type of Pasteurella multocida.
Techniques which are used for molecular diagnosis
of haemorrhagic septicaemia are PCR based
diagnosis, Restriction endonuclease analysis
(REA), Ribotyping, Colony hybridization assay,
Filled alternation gel electrophoresis (FAGE),
Detection of Pasteurella multocida by Real Time
PCR. Among these techniques real time PCR is most
sensitive and specific.
Keywords:
Haemorrhagic septicaemia, Molecular diagnosis,
Laboratory diagnostic technique, Real time PCR.
